MUTATION IN THE LYSA GENE IMPAIRS THE SYMBIOTIC PROPERTIES OF MESORHIZOBIUM CICERI.
Arch Microbiol. 2009 December 18;
Das SK, Gautam US, Sandhu KV, Bandyopadhyay S, Chakrabartty PK, Singh A
A Tn5-induced mutant of Mesorhizobium ciceri, TL28, requiring a amino poison lysine for expansion upon minimal middle was removed as well as characterized. A Tn5 insertion in a mutant aria TL28 was located upon a 6. 8-kb EcoRI bit of a chromosomal DNA. Complementation research with cloned DNA indicated which 1. 269 kb of DNA of a 6. 8-kb EcoRI bit easy a wild-type phenotype of a lysine-requiring mutant. This segment was serve characterized by DNA method research as well as was shown to enclose a coding method homologous to lysA gene of opposite germ. A lys( -) mutant TL28 was incompetent to bleed expansion of in effect nodules upon a roots of Cicer arietinum L. There was no detectable turn of lysine in a base exudates of chickpea. However, further of lysine to a plant expansion middle easy a capability of a mutant to furnish in effect nodules with nitrogen emplacement capability upon a roots of C. arietinum.
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